rabbit polyclonal anti actg2 antibodies  (Novus Biologicals)

Journal: BMC endocrine disorders

Article Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

doi: 10.1186/s12902-016-0100-3

Figure Lengend Snippet: Fig. 1 Analysis of ACTG2 protein expression in SI-NETs by immunohistochemistry (a-d) using ACTG2 antibody (NB100-91649 Novus Biologicals) and western blotting (e) using another ACTG2 antibody (TA313418 Origene). a Negatively stained tumor cells and strong stromal staining (20x). b Areas with positively stained tumor cells, and negative stromal staining (20x). c Weak staining in all tumor cells (20x). d No staining in absence of primary antibody (20x). e Western blotting analysis showing antibody specificity and correlation to immunohistochemistry analysis. One band only was visualized in two tumors (lanes 2 and 3) displaying strong stromal staining, and no band was detected in two tumors (lanes 1 and 4) with no staining in both tumor and stromal cells. Lane 1, lymph node metastasis; lanes 2–4, primary tumors. Coomassie blue staining was used as loading control, ladder in kDa

Article Snippet: The tissues were treated with normal serum from goat (S-1000, Vector) and two different rabbit polyclonal anti-ACTG2 antibodies (NB100-91649 Novus Biologicals, diluted 1/200, and TA313418 Origene, diluted 1/80) and anti-chromogranin A antibodies (Ab-1, LK2H10, NeoMarkers, diluted 1/1000) were used and incubated.

Techniques: Expressing, Immunohistochemistry, Western Blot, Staining, Control

Journal: BMC endocrine disorders

Article Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

doi: 10.1186/s12902-016-0100-3

Figure Lengend Snippet: Fig. 2 Double immunofluorescence staining of intestinal mucosa. Chromogranin A is visualized as green, showing positively stained enterochromaffin cells. ACTG2 is visualized as red and no staining is detected in chromogranin A positive cells (yellow)

Article Snippet: The tissues were treated with normal serum from goat (S-1000, Vector) and two different rabbit polyclonal anti-ACTG2 antibodies (NB100-91649 Novus Biologicals, diluted 1/200, and TA313418 Origene, diluted 1/80) and anti-chromogranin A antibodies (Ab-1, LK2H10, NeoMarkers, diluted 1/1000) were used and incubated.

Techniques: Double Immunofluorescence Staining, Staining

Journal: BMC endocrine disorders

Article Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

doi: 10.1186/s12902-016-0100-3

Figure Lengend Snippet: Fig. 3 Effects on ACTG2 mRNA expression in CNDT2.5 cells after DZNep (3-deazaneplanocin A) and 1.0 μM EPZ-6438 treatment, a and b respectively

Article Snippet: The tissues were treated with normal serum from goat (S-1000, Vector) and two different rabbit polyclonal anti-ACTG2 antibodies (NB100-91649 Novus Biologicals, diluted 1/200, and TA313418 Origene, diluted 1/80) and anti-chromogranin A antibodies (Ab-1, LK2H10, NeoMarkers, diluted 1/1000) were used and incubated.

Techniques: Expressing

Journal: BMC endocrine disorders

Article Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

doi: 10.1186/s12902-016-0100-3

Figure Lengend Snippet: Fig. 5 a miR-145 expression levels in 24 SI-NETs, and in CNDT2.5. A significant (p < 0.01) difference between primary tumors and liver metastases, and also between lymph node and liver metastasis (p < 0.001) was observed. A tendency towards decreased expression in lymph node metastases compared to primary tumors was detected (p = 0.09). b ACTG2 mRNA expression levels in 18 PT and 16 LNM. A significant (p < 0.01) difference between primary tumors and lymph node metastases was observed. PT, primary tumor. LNM, lymph node metastasis. LM, liver metastasis

Article Snippet: The tissues were treated with normal serum from goat (S-1000, Vector) and two different rabbit polyclonal anti-ACTG2 antibodies (NB100-91649 Novus Biologicals, diluted 1/200, and TA313418 Origene, diluted 1/80) and anti-chromogranin A antibodies (Ab-1, LK2H10, NeoMarkers, diluted 1/1000) were used and incubated.

Techniques: Expressing

Journal: BMC endocrine disorders

Article Title: A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis.

doi: 10.1186/s12902-016-0100-3

Figure Lengend Snippet: Fig. 6 a Colony formation assay in CNDT2.5 cells stably transfected with a plasmid expressing ACTG2 or with empty expression vector. b Western blotting demonstrating successful transfection of the DDK epitope fused to ACTG2. c Viability assay using WST-1 after transient overexpression of ACTG2. d Western blotting demonstrating successful transfection of the DDK epitope fused to ACTG2

Article Snippet: The tissues were treated with normal serum from goat (S-1000, Vector) and two different rabbit polyclonal anti-ACTG2 antibodies (NB100-91649 Novus Biologicals, diluted 1/200, and TA313418 Origene, diluted 1/80) and anti-chromogranin A antibodies (Ab-1, LK2H10, NeoMarkers, diluted 1/1000) were used and incubated.

Techniques: Colony Assay, Stable Transfection, Transfection, Plasmid Preparation, Expressing, Western Blot, Viability Assay, Over Expression